MULTIFUNCTIONAL PAMAM-SURFACE NANOSTRUCTURED PARTICLES ORGANIZED IN MULTIMERIC CLUSTERS. POTENTIAL SMART DELIVERY VEHICLES OF BIOACTIVE SPECIES THROUGH A HIGH SELECTIVE AMINE-THIOL BIOCONJUGATION STRATEGY

作者：Stancu I C^*; Lungu A; Rusen E; Mocanu A; Iordache P Z; Dragusin D M; Cotrut C; Vasile E; Iovu H

来源：Digest Journal of Nanomaterials and Biostructures, 2010, 5(4): 1077-1087.

• Clusters
• Dendrimers
• Nanorough surface
• Glutathione

The present work is devoted to the investigation of the polyamidoamine (PAMAM) dendrimer binding on the surface of polymer particles. Two generations of amino-terminal PAMAM macromolecules were chemically immobilized on the outer epoxy-shell of the polymer leading to nanostructured surface further available for cluster-like binding of a wide range of chemical species. The study proved that nanostructuring was successful and even enhanced by higher generation dendrimer. Multimeric clusters of functionalized particles were formed through physical interactions. Furthermore, the study was continued with the activation of the reactive amino-shell with a heterobifunctional reagent allowing the high-specific immobilization of thiol-containing species. A model thiol-ended peptide, glutathione, has been used to prove the efficiency of the cluster-type delivery system synthesized here. These materials open a generous route of creating solid surface nanostructured undermicronic particles decorated with chemically linked bioactive (and not only) molecules, to be further included in more complex scaffolds.

• 出版日期2010-12