A strategy for untargeted screening of macrolides and metabolites in bass by liquid chromatography coupled to quadrupole orbitrap mass spectrometry

作者：Jia, Wei^*; Shi, Lin; Chu, Xiaogang; Chang, James; Chen, Ying; Zhang, Feng

来源：Food Chemistry, 2018, 262: 110-117.

DOI：10.1016/j.foodchem.2018.04.090

• UHPLC/ESI Q-Orbitrap
• Macrolides
• Metabolites
• Untargeted screening

An analytical method for the non-target screening of macrolides and metabolites in bass (Lateolabrax) was developed using an automated on-line extraction procedure followed by ultrahigh-performance liquid chromatography coupled to electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC Q-Orbitrap). The estimated performance characteristics were satisfied, complying with the requirements of the guidelines specified in European Commission Decision 2002/657/EC. The decision limit ranged from 0.12 mu g kg(-1) to 3.61 mu g kg(-1), and detection capability ranged between 0.20 mu g kg(-1) and 6.02 mu g kg(-1). Precision in terms of relative standard deviation (RSD) was under 14% for all compounds, and the extraction recoveries ranged from 81% to 107%. Finally, the method was applied to ten different commercially important bass species and confirmed the presence of ten macrolides and metabolites. Five non-target compounds of robenidine, lincomycin hydrochloride, thiacloprid, fenbendazole, and thiabendazole were elucidated in the untargeted screening.

• 出版日期2018-10-1
• 单位中国检验检疫科学研究院; 陕西科技大学