A mass separation of chondrocytes from cartilage tissue utilizing an automatic crushing device

作者:Takagi Mutsumi*; Yoshioka Hiromichi; Wakitani Shigeyuki
来源:Journal of Bioscience and Bioengineering, 2010, 109(1): 73-74.
DOI:10.1016/j.jbiosc.2009.06.016

摘要

Porcine articular cartilage tissue was crushed using Multi-Beads-Shocker (TM) and digested with trypsin and collagenase type II. Chondrocyte cell yield was maximized by automatic crushing for 10 s, which was 4 times that by manual dicing with a surgical blade and might be useful for the automation of the cell processing.

  • 出版日期2010-1