Differential impact of oleate, palmitate, and adipokines on expression of NF-kappa B target genes in human vascular smooth muscle cells

作者：Lamers Daniela; Schlich Raphaela; Horrighs Angelika; Cramer Andrea; Sell Henrike; Eckel Juergen^*

来源：Molecular and Cellular Endocrinology, 2012, 362(1-2): 194-201.

DOI：10.1016/j.mce.2012.06.010

• Vascular smooth muscle cells
• Oleic acid
• Palmitic acid
• NF-kappa B
• Inflammation
• Vascular dysfunction

It is widely accepted that obesity is a major risk factor for the development of atherosclerosis. In this context, adipose tissue produces a variety of adipokines and releases free fatty acids, contributing to a chronic-low grade inflammation state implicated in vascular complications. In this study, we investigated the role of adipokines, oleic acid (OA), palmitic acid (PA), and the combinations on activation of NF-kappa B target genes in human vascular smooth muscle cells (SMC) to assess the hypothesis of synergistic interactions between these molecules. Adipocyte-conditioned medium (CM), generated from human adipocytes, in combination with low concentrations of OA, but not PA, induces SMC proliferation and activation of the transcription factor NF-kappa B in a synergistic way. Combined treatment of CM and OA further regulates a set of downstream NF-kappa B target genes including angiopoietin-1, activin A, and MMP-1, all critically involved in SMC dysfunction. This suggests that the lipotoxic potential of fatty acids is substantially enhanced by the presence of adipocyte-derived factors.

• 出版日期2012-10-15