科研之友
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摘要
This study presents findings on the protozoan Acanthamoeba in relation to its morphology, laboratory diagnosis, pathology in man, ecoepidemiological aspects and resistance to chlorine. Acanthamoeba was isolated from household water tanks (28.6%), from indoor swimming pools (71%), from the totality of samples collected from the Arroyo Naposta which crosses Bahia Blanca city, Argentina, whereas it was not detected in tap water. It was also isolated from a keratitis patient for the first time in Bahia Blanca. As to chlorine cysticidal action, growth was inhibited only after a 3 hour-exposure to 60 ppm chlorine. No further isolation of Acanthamoeba was perfomed in other biological samples. The ultrastructure of regional strains of Acanthamoeba was documented and molecular typification of a strain isolated from a keratitis patient was carried out. Although keratitis is a pathology of low prevalence, its ethiological diagnosis is low cost and easily executed, thus it is justified to rapidly and opportunely isolate Acanthamoeba from patients with this pathology. Direct observation sensitivity by optical microscopy reached 11.43% with respect to cultures. A list of recommendations was also prepared for contact lens wearers.
- 出版日期2010-9
