Study on dyeing wastewater treatment at high temperature by MBBR and the thermotolerant mechanism based on its microbial analysis

作者：Li, Chao; Zhang, Zhen; Li, Yi; Cao, Jiashun^*

来源：Process Biochemistry, 2015, 50(11): 1934-1941.

DOI：10.1016/j.procbio.2015.08.007

• Dyeing wastewater
• MBBR
• Microbial community
• EPS
• Thermotolerant mechanism

The dyeing wastewater treatment under increasing high temperature condition was studied in MBBRs. Results showed that as the temperature increase, the COD removal exhibited two best performances at 40 degrees C and 50 degrees C, and the maximum NH3-N removal was obtained at 35 degrees C and 40 degrees C The optimum temperature for extracellular polymeric substances (EPS) yield is 45 degrees C and the humic acid was the major contributor. Microbial community was analyzed by the association of DGGE, real-time PCR and high-throughput sequencing technologies. The highest abundance of AOB (characterized by amoA genes) was obtained at 35 degrees C, and it confirmed that the biofilm (attached on fillers) can facilitate the AOB abundance maintaining in the sludge. The "precursor and successor" thermophilic communities were enriched and dominant at different temperature stages, which mainly conclude genera Caldilinea (from 35 degrees C to 45 degrees C) and genera Rubellimicrobium and Pseudoxanthomonas (over 50 degrees C), respectively. It meant the thermophilic community displayed great evolution at the critical temperature "45 degrees C vertical bar 50 degrees C". Additionally, the process of thermotolerant mechanism establishment of the sludge in the MBBRs is proposed and the "two-stage enrichment" of different thermophilic communities was considered as the key procedure.

• 出版日期2015-11
• 单位河海大学