Ultra-trace arsenic and mercury speciation and determination in blood samples by ionic liquid-based dispersive liquid-liquid microextraction combined with flow injection-hydride generation/cold vapor atomic absorption spectroscopy

作者：Shirkhanloo Hamid; Khaligh Aisan; Mousavi Hassan Zavvar^*; Eskandari Mohammad Mehdi; Miran Beigi Ali Akbar

来源：CHEMICAL PAPERS, 2015, 69(6): 779-790.

DOI：10.1515/chempap-2015-0086

• arsenic
• mercury
• microwave assisted-UV system
• ionic liquid-based dispersive liquid-liquid microextraction
• hydride generation/cold vapor atomic absorption spectrometry

A simple, fast, and sensitive method for speciation and determination of As (III, V) and Hg (II, R) in human blood samples based on ionic liquid-dispersive liquid-liquid microextraction (IL-DLLME) and flow injection hydride generation/cold vapor atomic absorption spectrometry (FI-HG/CV-AAS) has been developed. Tetraethylthiuram disulfide, mixed ionic liquids (hydrophobic and hydrophilic ILs) and acetone were used in the DLLME step as the chelating agent, extraction and dispersive solvents, respectively. Using a microwave assisted-UV system, organic mercury (R-Hg) was converted to Hg(II) and total mercury amount was measured in blood samples by the presented method. Total arsenic content was determined by reducing As(V) to As(III) with potassium iodide and ascorbic acid in a hydrochloric acid solution. Finally, As(V) and R-Hg were determined by mathematically subtracting the As(III) and Hg(II) content from the total arsenic and mercury, respectively. Under optimum conditions, linear range and detection limit (3 sigma) of 0.1-5.0 mu g L-1 and 0.02 mu g L-1 for As(III) and 0.15-8.50 mu g L-1 and 0.03 mu g L-1 for Hg(II) were achieved, respectively, at low RSD values of < 4 % (N = 10). The developed method was successfully applied to determine the ultra-trace amounts of arsenic and mercury species in blood samples; the validation of the method was performed using standard reference materials.

• 出版日期2015-6