Upregulation of NaV1.7 in Dorsal Root Ganglia After Intervertebral Disc Injury in Rats

作者：Sadamasu Aya; Sakuma Yoshihiro; Suzuki Miyako; Orita Sumihisa; Yamauchi Kazuyo; Inoue Gen; Aoki Yasuchika; Ishikawa Tetsuhiro; Miyagi Masayuki; Kamoda Hiroto; Kubota Gou; Oikawa Yasuhiro; Inage Kazuhide; Sainoh Takeshi; Sato Jun; Nakamura Junichi; Toyone Tomoaki; Takahashi Kazuhisa; Ohtori Seiji^*

来源：Spine, 2014, 39(7): E421-E426.

DOI：10.1097/BRS.0000000000000229

• NaV1.7
• dorsal root ganglion
• intervertebral disc
• injury
• rat

Study Design. Animal study. %26lt;br%26gt;Objective. To investigate pain-related expression of NaV1.7 in dorsal root ganglia (DRG) innervating intervertebral discs. %26lt;br%26gt;Summary of Background Data. The pathophysiology of discogenic low back pain is not fully understood. Prostaglandins and cytokines produced by degenerated discs can cause pain, but nonsteroidal anti-inflammatory and steroid medications are often ineffective at pain reduction. Tetrodotoxin-sensitive, voltage-gated sodium (NaV) channels are associated with sensory transmission in primary sensory nerves, and the NaV1.7 channel has emerged as an attractive analgesic target. The purpose of this study was to investigate pain-related expression of NaV1.7 in DRG innervating intervertebral discs. %26lt;br%26gt;Methods. Using a rodent model of disc puncture, we labeled DRG neurons innervating L5-L6 discs with FluoroGold neurotracer (n = 20). Half of the rats (n = 10) underwent intervertebral disc puncture using a 23-gauge needle (puncture group), and the other half underwent non-puncture sham surgery (non-puncture group). Seven and 14 days after surgery, DRGs from the L1 to L6 levels were harvested, sectioned, and immunostained for NaV1.7, and the proportion of NaV1.7-immunoreactive DRG neurons was evaluated. %26lt;br%26gt;Results. NaV1.7 was expressed in DRG neurons innervating intervertebral discs from L1 to L5. The ratio of NaV1.7-expressing DRG neurons to total FG-labeled neurons was 7.2% and 7.6% at 1 and 2 weeks after surgery, respectively, in the non-puncture group and 16.2% and 16.3% at 1 and 2 weeks, respectively, in the puncture group. The upregulation of NaV1.7 after puncture was significant at both 1 and 2 weeks after surgery (P %26lt; 0.01). %26lt;br%26gt;Conclusion. We found that disc injury increases NaV1.7 expression in DRG neurons innervating injured discs. NaV1.7 may be a therapeutic target for pain control in patients with lumbar disc degeneration.

• 出版日期2014-4-1