Assessment of the ability of five culture media for the detection of Escherichia coli O157

作者：Gutierrez Myriam E; Janes Marlene E^*; Torrico Damir D; Carabante Kennet M; Prinyawiwatkul Witoon

来源：International Journal of Food Science and Technology, 2016, 51(8): 1910-1915.

DOI：10.1111/ijfs.13164

• Cattle
• CHROMagar
• environmental samples
• Escherichia coli O157
• Sorbitol MacConkey Agar

Cattle are a common reservoir for Escherichia coli O157:H7. Prior to confirming its presence in a sample, proper isolation of E.coli O157 is necessary. Consequently, this study evaluated the ability of five commercial plating media to isolate E.coli O157 from 138 samples of fresh cattle faeces, water from water trough and pond, and surfaces of water trough and hay bunk. For the isolation of E.coli O157, samples were enriched in tryptic soya broth, followed by immunoseparation and then plating on SMAC, CT-SMAC, CHROMagar O157, Tellurite CHROMagar O157 and Vancomycin Cefixime Cefsoludin CHROMagar O157. Real-time PCR targeting genes stx(1), stx(2) and wzy(O157) was used to confirm selected isolates. When analysed together, CT-SMAC and CHROMagar O157 were the best combination for isolating E.coli O157, giving 79% true-positive results and only 0.05% false-negative results.

• 出版日期2016-8