Acute Toxicity Study of Zerumbone-Loaded Nanostructured Lipid Carrier on BALB/c Mice Model

作者：Rahman Heshu Sulaiman^*; Rasedee Abdullah; Othman Hemn Hassan; Chartrand Max Stanley; Namvar Farideh; Yeap Swee Keong; Samad Nozlena Abdul; Andas Reena Joys; Nadzri Nabilah Muhammad; Anasamy Theebaa; Ng Kuan Beng; How Chee Wun

来源：Biomed Research International, 2014, 2014: 563930.

DOI：10.1155/2014/563930

Zerumbone-(ZER-) loaded nanostructure lipid carrier (NLC) (ZER-NLC) prepared for its antileukemia effect in vitro was evaluated for its toxicological effects by observing changes in the liver, kidney, spleen, lung, heart, and brain tissues, serum biochemical parameters, total haemogram, and bone marrow stem cells. The acute toxicity study for ZER-NLC was conducted by orally treating BALB/c mice with a single dose with either water, olive oil, ZER, NLC, or ZER-NLC for 14 days. The animals were observed for clinical and behavioral abnormalities, toxicological symptoms, feed consumption, and gross appearance. The liver, kidney, heart, lung, spleen, and brain tissues were assessed histologically. Total haemogram was counted by hemocytometry and microhematocrit reader. Bone marrow examination in terms of cellular morphology was done by Wright staining with bone marrow smear. Furthermore, serum biochemical parameters were determined spectrophotometrically. Grossly all treated mice, their investigated tissues, serum biochemical parameters, total haemogram, and bone marrow were normal. At oral doses of 100 and 200 mg/kg ZER-NLC there was no sign of toxicity or mortality in BALB/c mice. This study suggests that the 50% lethal dose (LD50) of ZER-NLCis higher than 200 mg/kg, thus, safe by oral administration.

• 出版日期2014
• 单位中国林业科学研究院热带林业研究所