A DNA PRIMER PROBE SYSTEM FOR THE RAPID AND SENSITIVE DETECTION OF MYCOBACTERIUM-TUBERCULOSIS-COMPLEX PATHOGENS

作者:RALPHS NT*; GARRETT S; MORSE R; COOKSON JB; ANDREW PW; BOULNOIS GJ
来源:Journal of Applied Bacteriology, 1991, 70(3): 221-226.
DOI:10.1111/j.1365-2672.1991.tb02928.x

摘要

BamHI restriction fragment from Mycobacterium tuberculosis was found to hybridize specifically with genomic DNA from M. tuberculosis-complex organisms. Primers were designed from the terminal sequences of this fragment and used to amplify uniquely M. tuberculosis-group DNA in a polymerase chain reaction. It is suggested that a combination of these primers and probe will prove a useful tool for the early diagnosis of tuberculosis infections.

  • 出版日期1991-3