A novel structural fucosylated chondroitin sulfate from Holothuria Mexicana and its effects on growth factors binding and anticoagulation

作者：Li, Qinying; Cai, Chao; Chang, Yaoguang; Zhang, Fuming; Linhardt, Robert J.; Xue, Changhu; Li, Guoyun^*; Yu, Guangli^*

来源：Carbohydrate Polymers, 2018, 181: 1160-1168.

DOI：10.1016/j.carbpol.2017.10.100

• Fucosylated chondroitin sulfate
• Holothuria mexicana
• Structure elucidation
• Anti-angiogenesis
• Anticoagulant

Fucosylated chondroitin sulfate (FCS), a structurally distinct glycosaminoglycan from the body wall of sea cucumber, possesses many biological properties and pharmacology functions. The refined structure of FCS isolated from sea cucumber Holothuria Mexicana (FCShm) was characterized by NMR spectra and HILIC-FTMS, which demonstrated four types of branches in FCShm. Among these, two branches were alpha-L-Fuc-2S4S (where Fuc is fucose and S is sulfo) and alpha-L-Fuc-4S linked to O-3 of glucuronic acid residues, while others were identified as alpha-L-Fuc-4S and alpha-L-Fuc-3S4S attached to O-6 of N-acetylgalactosamine residue. Furthermore, the fucosyl branches were alpha-1,3-linked with different degree of polymerization from 1 to 5. FCShm exhibited high affinity to fibroblast growth factor 1 and 2, growth factors involved in neovascularization. Moreover, FCShm displayed intrinsic anticoagulant activity and inhibited thrombin and factor Xa activation by antithrombin III. Our results proposed a novel structural FCS and demonstrated its favorable application prospects in anti-angiogenesis and anticoagulation.

• 出版日期2018-2-1
• 单位中国海洋大学