Atomic Force Microscopy Study of Ganglioside GM1 Concentration Effect on Lateral Phase Separation of Sphingomyelin/Dioleoylphosphatidylcholine/Cholesterol Bilayers

作者：Bao Ren; Li Li; Qiu Feng^*; Yang Yuliang

来源：Journal of Physical Chemistry B, 2011, 115(19): 5923-5929.

DOI：10.1021/jp2008122

The effect of monosialoganglioside GM1 (GM1) concentration on the lateral phase separation in the sphingomyelin/1,2-dioleoyl-sn-glycero-3-phosphocholine/cholesterol (SM/DOPC/Chol) bilayers was studied by using atomic force microscopy. The results show that, with the increase of GM1 mol fraction (x), the dominant composition of liquid-ordered (L(o)) domains changes from SM to SM/GM1 and finally to GM1. Meanwhile, the decrease of domain area (A) of the L(o) phase with the increase of x follows a scaling law of A similar to x (-3/2), for x > 0.005, indicating that the domain growth is pinned with high GM1 concentration. Results of in situ experiments of GM1 insertion into SM/DOPC/cholesterol bilayers further supported our observations.

• 出版日期2011-5-19
• 单位复旦大学