科研之友
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摘要
The objective of the current investigation was to assess the antiproliferative activity of campesterol in HCT-116 human colon carcinoma cells and to evaluate its effect on apoptosis induction, cell migration and mitochondrial membrane potential loss (AIM). The cytotoxic effect of campesterol was evaluated by sulforhodamine B (SRB) as well as clonogenic assays. Its effect on apoptosis was studied by fluorescence and transmission electron microscopic techniques. In vitro wound healing assay using inverted light microscope was used to evaluate its effect on cell migration while as flow cytometry was used for assessing its effect on A psi m. Cell viability assay results show that campesterol exhibits significant cytotoxic effect in HCT-116 cells indicating IC50 values of 45.5, 32.2, and 22.4 mu M at 24, 48, and 72 h time intervals, respectively. Fluorescence microscopy and TEM results indicate that campesterol induces early as well as late apoptotic effects in these cell lines characterized by chromatin condensation, membrane blebbing, apoptotic body formation, appearance of vacuoles, swelling of mitochondria and endoplasmic reticulum. Campesterol also induced dose-dependent loss of mitochondrial membrane potential as well as suppression of cancer cell migration.
