A novel approach obtaining intron-containing hairpin RNA constructs

作者：Wen Chun Miao; Cai Ping Zhong^*; Xu Zheng Jun; Zhong Wan Fang

来源：Bioscience Biotechnology and Biochemistry, 2008, 72(2): 615-617.

DOI：10.1271/bbb.70616

• intron-containing hairpin RNA
• genomic DNA
• recombinant-PCR
• Zea mays
• auxin-binding protein

Recently, much research on constitutive expression of an intron-containing self-complementary hair-pin RNA (ihpRNA) have been reported to silence target genes efficiently in a variety of species. Here we designed a new recombinant-PCR mediated method called direct amplification of ihpRNA from genomic DNA. This approach has proved to be easy, stable, and efficient.

• 出版日期2008-2
• 单位四川省农业科学院