Determination of Mycotoxins in Bee Pollen by Gas Chromatography-Tandem Mass Spectrometry

作者：Rodriguez Carrasco Yelko; Font Guillermina; Manes Jordi; Berrada Houda^*

来源：Journal of Agricultural and Food Chemistry, 2013, 61(8): 1999-2005.

DOI：10.1021/jf400256f

• bee pollen
• mycotoxins
• QuEChERS
• GC-MS/MS
• triple quadrupole

Bee pollen, promoted as a natural food supplement, is consumed increasingly by people to maintain a healthy diet. Depending on environmental conditions, pollen can also be an optimum medium for growth of molds such as Fusarium and Penicillium. A quick, easy, cheap, rapid, and safe (QuEChERS) extraction procedure followed by a gas chromatography-tandem mass spectrometry (GC-MS/MS) determination of eight selected Fusarium toxins in bee pollen was developed and optimized. Recovery studies at 20, 80, and 1000 mu g/kg showed values between 73 and 95% with relative standard deviations (RSDs) of %26lt;15% for all studied mycotoxins. Limits of quantitation (LOQ) ranged from 1 to 4 mu g/kg. The proposed method was applied to the analysis of 15 commercial samples. Two of 15 samples showed quantifiable values for neosolaniol and nivalenol.

• 出版日期2013-2-27