A microsystem integrating photodegradable hydrogel microstructures and reconfigurable microfluidics for single-cell analysis and retrieval

作者：Son Kyung Jin; Shin Dong Sik; Kwa Timothy; You Jungmok; Gao Yandong; Revzin Alexander^*

来源：Lab on a Chip, 2015, 15(3): 637-641.

DOI：10.1039/c4lc00884g

We developed a micropatterned photodegradable hydrogel array integrated with reconfigurable microfluidics to enable cell secretion analysis and cell retrieval at the single-cell level. The activity of protease molecules secreted from single cells was monitored using FRET peptides entrapped inside microfabricated compartments. Antibody-modified gel islands tethering cells to the surface could be degraded by UV exposure to release specific single cells of interest.

• 出版日期2015