An efficient adventitious shoot regeneration system for ramie (Boehmeria nivea Gaud) using thidiazuron

作者：Wang Bo; Peng Dingxiang^*; Liu Lijun; Sun Zhenxia; Zhang Na; Gao Shimei

来源：Botanical Studies, 2007, 48(2): 173-180.

• Boehmeria nivea Gaud
• cotyledon
• organogenesis
• ramie
• thidiazuron

An efficient culture system for direct adventitious shoot regeneration of plantlet from cotyledon of ramie (Boehmeria nivea Gaud) was established and factors affecting shoot regeneration efficiencies were evaluated. Cotyledons excised from 4-day-old seedlings showed the highest regeneration capacity. Various concentrations of thidiazuron (TDZ) and indoleacetic acid (IAA) gave rise to adventitious shoots with different efficiencies. The cultures of cotyledons from 4-day-old ramie seedlings on MS medium supplemented with 2.27 mu M TDZ and 0.057 mu M IAA were shown to have the highest efficiency (83.6%) of shoot regeneration among the four auxin supplements (2,4-D; IAA; IBA; NAA). Regeneration of shoot in six cultivars was genotype-dependent, '5041-3' being the most responsive one. The regenerated shoots were transferred to hormone-free medium for shoot elongation, after successfully rooted on half-strength MS medium supplemented with 0.27 mu M NAA. The rooted plantlets with 4-5 leaves were transplanted to greenhouse for further growth. To our knowledge, this is the first report on efficient regeneration of plantlet from cotyledon of ramie, which will be of value for genetic improvement in the near future.

• 出版日期2007-4
• 单位华中农业大学