Augmentation of CCL17 and CCL28 gene expression by TNF-alpha, IL-1 beta, or IFN-gamma in cultured canine keratinocytes

作者：Shibata Sanae; Maeda Sadatoshi; Maeda Shingo; Chimura Naoki; Kondo Naho; Fukata Tsuneo

来源：Research in Veterinary Science, 2010, 88(3): 422-426.

DOI：10.1016/j.rvsc.2009.11.011

• Dogs
• Atopic dermatitis
• Keratinocytes
• Pro-inflammatory cytokines
• CCL17/TARC
• CCL28/MEC

Keratinocytes produce inflammatory mediators that are involved in the pathogenesis of skin disorders such as atopic dermatitis (AD). In particular, the CC chemokines, thymus and activation regulated chemokine (TARC)/CCL17 and mucosae-associated epithelial chemokine (MEC)/CCL28 are considered to play an important role in the lesional infiltration of lymphocytes in canine AD. However, there have been no reports on the regulatory mechanisms of CCL17 and CCL28 transcription in canine keratinocytes. In this study, we investigated whether CCL17 and CCL28 transcription in cultured keratinocytes is induced by TNF-alpha, IL-1 beta, or IFN-gamma. It was found that CCL17 mRNA transcription is augmented by TNF-a only, whereas the CCL28 mRNA level could be increased by TNF-alpha, IL-1 beta, or IFN-y. The present study suggests that proinflammatory cytokines are important inducing factors for the production of CCL17 and CCL28 in the lesional skin of dogs with AD.

• 出版日期2010-6