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摘要
The effect of the addition of maltitol on the production of raw starch digesting glucoamylase (RSDG) by Aspergillus niger F-08 was studied in the paper. The activity of RSDG formed by Aspergillus niger F-08 was enhanced dramatically by the addition of maltitol to the medium and it was confirmed that maltitol acted as a very efficient inducer for RSDG production. As a result, with the addition of 8 g maltitol in 1 l basal medium at the beginning of the culture, the enzyme activity was enhanced near 8-fold. The synthesis mechanism of RSDG by Aspergillus niger F-08 was also studied on molecular level. It was found that the regulation of RSDG synthesis by Aspergillus niger F-08 occurred at both transcriptional and translational level and the enzyme synthesis was provoked by the addition of maltitol at transcriptional level.
- 出版日期2008-11
- 单位中国热带农业科学院
