Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE)

作者：Deutsch Eric W^*; Ball Catherine A; Berman Jules J; Bova G Steven; Brazma Alvis; Bumgarner Roger E; Campbell David; Causton Helen C; Christiansen Jeffrey H; Daian Fabrice; Dauga Delphine; Davidson Duncan R; Gimenez Gregory; Goo Young Ah; Grimmond Sean; Henrich Thorsten; Herrmann Bernhard G; Johnson Michael H; Korb Martin; Mills Jason C; Oudes Asa J; Parkinson Helen E; Pascal Laura E; Pollet N Icolas; Quackenbush John; Ramialison Mirana; Ringwald Martin

来源：Nature Biotechnology, 2008, 26(3): 305-312.

DOI：10.1038/nbt1391

One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.

• 出版日期2008-3
• 单位河北医科大学; 上海生物信息技术研究中心