Miniaturized hollow fiber assisted liquid-phase microextraction with in situ derivatization and gas chromatography-mass spectrometry for analysis of bisphenol A in human urine sample

作者：Kawaguchi Migaku; Ito Rie; Okanouchi Noriya; Saito Koichi; Nakazawa Hiroyuki^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2008, 870(1): 98-102.

DOI：10.1016/j.jchromb.2008.06.011

• bisphenol A (BPA)
• miniaturized
• hollow fiber (HF)
• liquid-phase microextraction (LPME)
• GC-MS
• in situ derivatization

A new method that involves miniaturized hollow fiber assisted liquid-phase microextraction (HF-LPME) with in situ derivatization and gas chromatography-mass spectrometry (GC-MS) is described for the determination of trace amounts of bisphenol A (BPA) in human urine samples. The detection limit and the quantification limit of BPA in human urine sample are 0.02 and 0.1 ng ml(-1) (ppb), respectively. The calibration curve for BPA is linear with a correlation coefficient of >0.999 in the range of 0.1-50 ng ml(-1). The average recoveries of BPA in human urine samples spiked with 1 and 5 ng ml(-1) BPA are 101.0 (R.S.D.: 6.7%) and 98.8 (R.S.D.: 1.8%), respectively, with correction using the added surrogate standard, bisphenol A-C-13(12). This simple, accurate, sensitive and selective analytical method can be applicable to the determination of trace amounts of BPA in human urine samples.

• 出版日期2008-7-1