Alterations in Antibody Subclass Immune Reactivity to Trophoblast-derived Fetal Fibronectin and alpha 2-Macroglobulin in Women with Recurrent Pregnancy Loss

作者：Saunders Rhiana D^*; Nakajima Steven T; Rai Shesh N; Pan Jianmin; Gercel Taylor Cicek; Taylor Douglas D

来源：American Journal of Reproductive Immunology, 2012, 68(5): 438-449.

DOI：10.1111/j.1600-0897.2012.01182.x

• Antibody reactivity
• humoral immunity
• recurrent pregnancy loss
• trophoblast

Problem Increasing evidence supports the involvement of complex antibody-mediated immunologic events at the deciduatrophoblast interface. Our objective is to define the humoral immune responses of pregnant women with a history of recurrent pregnancy loss (RPL) compared with gestation-age-matched and non-pregnant controls in terms of trophoblast-derived antigenic targets and IgG subclasses. Method of study Immunoprecipitation and Western immunoblotting were performed to characterize IgG subclass reactivity to Sw.71 trophoblastderived fetal fibronectin and alpha-2-macroglobulin, using serum obtained from first-trimester pregnant RPL subjects, gestation-age-matched controls, and non-pregnant controls. Results Using a generalized linear model, sera from women with a history of RPL exhibited increased IgG3 immunoreactivity to trophoblast-derived fetal fibronectin and alpha-2-macroglobulin compared with controls (P%26lt;0.001 and P%26lt;0.001, respectively). Conclusion IgG3 reactivity in women with RPL may play a significant role in aberrant immune-regulatory mechanisms in early pregnancy. Further investigations into the role of autoantibodies against trophoblast-derived proteins in implantation and pregnancy are warranted.

• 出版日期2012-11