Analysis of hOGG1 genotype as a prognostic marker for muscle invasive bladder cancer: A novel approach using peptide nucleic acid-mediated, real-time PCR clamping

作者：Kim Eun Jung; Yan Chunri; Ha Yun Sok; Jeong Pildu; Kim Isaac Yi; Moon Sung Kwon; Choi Yung Hyun; Kim Wun Jae^*

来源：Urologic Oncology-Seminars and Original Investigations, 2012, 30(5): 673-679.

DOI：10.1016/j.urolonc.2010.07.008

• Urinary bladder neoplasms
• DNA damage repair mechanisms
• hOGG1
• Biological tumor markers
• Prognosis
• PNA

Objective: DNA damage repair mechanisms are a source of genetic mutation and are believed to play an important role in human cancer. Human 8-oxoguanine DNA glycosylase I (hOGG1) is involved in the recognition and repair of DNA damage. The value of the hOGG1 genotype as a prognostic indicator for bladder cancer (BC) was assessed using a novel technological approach. %26lt;br%26gt;Materials and methods: The association between genetic polymorphism of hOGG1 codon 326 and clinicopathologic characteristics of 337 patients with BC was analyzed using peptide nucleic acid (PNA)-mediated real-time PCR clamping. %26lt;br%26gt;Results: Tumor grade and size were significantly associated with the hOGG1 codon 326 genotype in non-muscle-invasive bladder cancer (NMIBC). The Cys326Cys polymorphism was significantly associated with progression and cancer specific survival in patients with muscle-invasive bladder cancer (MIBC). Multivariate Cox regression analysis indicated that the hOGG1 Cys326Cys polymorphism is associated with a protective effect on progression and a more dominant survival benefit than the Ser326Ser polymorphism in MIBC (hazard ratio 0.284 and 0.305, respectively). %26lt;br%26gt;Conclusions: Analysis of genotypes and clinical data for 337 BC patients indicates that the hOGG1 genotype may be a useful prognostic genetic marker for MIBC.

• 出版日期2012-10