ASSESSMENT OF CARBON SOURCES ON IN VITRO SHOOT REGENERATION IN TOMATO

作者：Shah Sabir Hussain; Ali Shaukat^*; Jan Sohail Ahmad; Ali Ghulam Muhammad

来源：Pakistan Journal of Agricultural Sciences, 2014, 51(1): 197-207.

• Hypocotyls
• in vitro regeneration
• explant
• sucrose
• sorbitol
• Solanum lycopersicum

An innovative approach for in vitro shoot regeneration by both direct and indirect means was developed in three tomato genotypes culturing hypocotyls and leaf discs explants on MS and N6 basal media fortified with various concentrations of carbon sources (sucrose and sorbitol) individually, accumulatively and also in amalgamation with various plant growth regulators. No response of in vitro shoot regeneration was recorded in all the genotypes by the individual application of carbon sources in both MS and N6 basal media. On the other hand, their accumulative effect rapidly enhanced the in vitro shoot regeneration frequency in all the genotypes. The highest shoot organogenesis frequency (100, 99.00 and 97.69%) was recorded in Rio Grande, Roma and Moneymaker, respectively on MS medium fortified with carbon sources (30: 30 g/l) culturing hypocotyls. Supplementation of sucrose: sorbitol (30: 30 g/l) in N6 medium along with different PGRs (0.1 mg/l IAA, 1.0 mg/l ZEA and 2.0 mg/l BAP) produced the highest shoot regeneration frequency (96.33, 92.69 and 88.74%) in Roma, Rio Grande and Moneymaker culturing leaf discs. Our findings suggest an alternative approach as hormone- free protocol for in vitro shoot regeneration in tomato that would save the resources with regard to hormonal costs and time.

• 出版日期2014-3