Mutasynthesis of Lincomycin Derivatives with Activity against Drug-Resistant Staphylococci

作者：Ulanova Dana^*; Novotna Jitka; Smutna Yvona; Kamenik Zdenek; Gazak Radek; Sulc Miroslav; Sedmera Petr; Kadlcik Stanislav; Plhackova Kamila; Janata Jiri

来源：Antimicrobial Agents and Chemotherapy, 2010, 54(2): 927-930.

DOI：10.1128/AAC.00918-09

The lincomycin biosynthetic gene lmbX was deleted in Streptomyces lincolnensis ATCC 25466, and deletion of this gene led to abolition of lincomycin production. The results of complementation experiments proved the blockage in the biosynthesis of lincomycin precursor 4-propyl-L-proline. Feeding this mutant strain with precursor derivatives resulted in production of 4'-butyl-4'-depropyllincomycin and 4'-pentyl-4'-depropyllincomycin in high titers and without lincomycin contamination. Moreover, 4'-pentyl-4'-depropyllincomycin was found to be more active than lincomycin against clinical Staphylococcus isolates with genes determining low-level lincosamide resistance.

• 出版日期2010-2