Designing broad-spectrum anti-HIV-1 gRNAs to target patient-derived variants

作者：Dampier Will; Sullivan Neil T; Chung Cheng Han; Mell Joshua Chang; Nonnemacher Michael R; Wigdahl Brian^*

来源：Scientific Reports, 2017, 7(1): 14413.

DOI：10.1038/s41598-017-12612-z

Clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated protein 9 (Cas9), including specific guide RNAs (gRNAs), can excise integrated human immunodeficiency virus type 1 (HIV-1) provirus from host chromosomes. To date, anti-HIV-1 gRNAs have been designed to account for off-target activity, however, they seldom account for genetic variation in the HIV-1 genome within and between patients, which will be crucial for therapeutic application of this technology. This analysis tests the ability of published anti-HIV-1 gRNAs to cleave publicly available patient-derived HIV-1 sequences to inform gRNA design and provides basic computational tools to researchers in the field.

• 出版日期2017-10-31