Antisense oligonucleotide targeting midkine suppresses in vivo angiogenesis

作者：Dai, Li-Cheng^*; Wang, Xiang; Yao, Xing; Lu, Yong-Liang; Ping, Jin-Liang; He, Jian-Fang

来源：World Journal of Gastroenterology, 2007, 13(8): 1208-1213.

DOI：10.3748/wjg.v13.i8.1208

• midkine
• angiogenesis
• antisense oligonucleotide
• tumor
• chick chorioallantoic membrane assay
• hepatocellular carcinoma

AIM: To evaluate the effect of antisense oligonucleotide targeting midkine (MK-AS) on angiogenesis in chick chorioallantoic membrane (CAM) and in situ human hepatocellular carcinoma (HCC).
METHODS: An in situ human hepatocellular carcinoma (HCC) model and CAM assay were used in this experiment. The effect of MK-AS on angiogenesis was evaluated by cell proliferation assay and hematoxylineosin (HE) staining.
RESULTS: MK-AS significantly inhibited human umbilical vein endothelial cells (HUVEC) and in situ human HCC growth. At the same time, MK-AS suppressed the angiogenesis both in human hepatocellular carcinoma cell line (HEPG2)-induced CAM and in situ human HCC tissues.
CONCLUSION: MK-AS is an effective antiangiogenesis agent in vivo.

• 出版日期2007-2-28
• 单位湖州市中心医院