A new PPARy/DNA origami biochromatography and offline high performance liquid chromatography-mass spectrometry method for screening PPAR gamma receptor antagonists from ginsenosides

作者：Zhou, Jie^*; Sun, Chong; Meng, Lingchang; Ye, Weiran; Luo, Pei; Sun, Fang; Chen, Shanshan; Xu, Xia^*

来源：Archives of Biochemistry and Biophysics, 2017, 629: 63-69.

DOI：10.1016/j.abb.2017.07.010

• PPAR gamma
• Biochromatography
• HPLC/MS
• PPAR gamma antagonists
• Ginsenosides

To rapidly identify novel PPAR gamma ligands, a robust binding assay amenable to high-efficiency screening toward PPARy would be desirable. In this study, a new PPAR gamma assembled on DNA origami (PPAR gamma/DNA origami) biochromatography drug screening model was constructed and evaluated. The method was used to screen active ingredients acted on PPAR gamma from the total ginsenosides. The total ginsenosides were handled on this biochromatography column by HPLC. The collected retention fraction from the biochromatography column was analyzed by HPLC and HPLC/MS. The results showed that ginsenoside Re from the total ginsenosides was the targeted component which could act on PPAR gamma receptor in similar manner of rosiglitazone as a control drug. This method will be a useful method for drug screening with natural medicinal herbs as a leading compound resource, compared with previous drug screening, this method without the need for complex and time-consuming separation steps previously.

• 出版日期2017-9-1
• 单位郑州大学