Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues

作者:Pereira Lara E; Makarova Natalia; Dobard Charles; Aubert Rachael D; Srinivasan Priya; McNicholl Janet; Smith James M*
来源:Journal of Medical Primatology, 2014, 43(5): 360-363.
DOI:10.1111/jmp.12121

摘要

Background and Methods Cell isolation from macaque tissues involves laborious enzymatic digestion. The Medimachine provides a simpler, quicker nonenzymatic method, yielding 1.5-5 million cells/g of vaginal or rectal tissue from pigtailed macaques. Results and Conclusions Flow cytometry analysis of the two methods revealed similar levels of cell viability and most major cell lineage and activation markers.

  • 出版日期2014-10

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