Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe

作者：Zhang Jiangyan; Li Zhengping^*; Wang Hui; Wang Yucong; Jia Hongxia; Yan Jingli

来源：Chemical communications, 2011, 47(33): 9465-9467.

DOI：10.1039/c1cc13466c

It was first demonstrated that the DNA probe modified with ribonucleotides can be efficiently ligated by using miRNA as the template. With PCR amplification of the ligated DNA probe, as low as 0.2 fM target miRNAs can be detected with high specificity.

• 出版日期2011
• 单位河北大学