A rapid DNA extraction method for PCR amplification from wetland soils

作者：Li, J.; Li, B.; Zhou, Y.; Xu, J.; Zhao, J.^*

来源：Letters in Applied Microbiology, 2011, 52(6): 626-633.

DOI：10.1111/j.1472-765X.2011.03047.x

• ammonia-oxidizing archaea
• ammonia-oxidizing bacteria
• calcium chloride
• glass bead
• SDS method

Aims:
We tested a method of rapid DNA extraction from wetland soil samples for use in the polymerase chain reaction.
Methods and Results:
The glass bead/calcium chloride/SDS method obtained in the present study was compared with the calcium chloride/SDS/enzymatic extraction method and the UltraClean (TM) Soil DNA Isolation Kit. Rapid DNA extraction could be completed within about two hours without purification steps.
Conclusions:
This study succeeded in establishing a fast soil DNA extraction protocol that can be applied to various environmental sources that are rich in humic acid content.
Significance and Impact of the Study:
The method provides a technology with high-quality DNA extraction from soils for testing the diversity of AOB and AOA.

• 出版日期2011-6
• 单位内蒙古大学