Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library

作者：Dantas Barbosa Carmela^*; Faria Fabricia P; Brigido MarceloM; Maranhao Andrea Q

来源：Journal of Biomedicine and Biotechnology, 2009, 2009: 157531.

DOI：10.1155/2009/157531

Osteosarcoma, a highly malignant disease, is the most common primary bone tumor and is frequently found in children and adolescents. In order to isolate antibodies against osteosarcoma antigens, a combinatorial osteosarcoma Fab library displayed on the surface of phages was used. After three rounds of selection on the surface of tumor cells, several osteosarcoma-reactive Fabs were detected. From these Fabs, five were better characterized, and despite having differences in their VII (heavy chain variable domain) and V kappa (kappa chain variable domain) regions, they all bound to a protein with the same molecular mass. Further analysis by cell ELISA and immunocytochemistry suggested that the Fabs recognize a membrane-associated tumor antigen expressed in higher amounts in neoplasic cells than in normal tissue. These results suggest that the human Fabs selected in this work are a valuable tool for the study of this neoplasia.

• 出版日期2009