A latex agglutination test for the field determination of abnormal vitellogenin production in male fishes contaminated by estrogen mimics

作者：Magalhaes I; Pihan JC; Falla J^*

来源：Journal of Physics: Condensed Matter , 2004, 16(22): S2167-S2175.

DOI：10.1088/0953-8984/16/22/016

Estrogen mimics are pollutants present in the aquatic environment. These compounds induce abnormalities in the reproductive system of male fishes, which lead to a total or partial male feminization, or to their demasculinization. Ultimately, these alterations could lead to a disappearance of the total contaminated fish population. Moreover, these toxic substances possess the capacity to mimic endogenous estrogens and to induce the abnormal production of vitellogenin (VTG) in male and immature fishes. The purpose of this research was to develop an easy, specific, cheap and fast method for diagnosing the contamination of male fishes by estrogen mimics, using VTG as biomarker. The selected method is based on a reverse latex agglutination test (rLAT), developed with monoclonal antibodies specific of this biomarker. The development of this VTG-rLAT has involved, firstly, the purification of carp VTG to produce monoclonal antibodies, specifics of this protein. One of these antibodies was selected to recover latex particles (diameter: 1 mum). Finally, the immunoreactivity of the VTG-rLAT was verified with different fish plasma samples from males treated with 17beta-estradiol and non-treated males or females in vitellogenesis.

• 出版日期2004-6-9