A Simple Method for Controlling the Degree of PEGylation of Dynorphin A (1-13)

作者：Wang Xiufang; Tian Yong^*; Zhong Guoying; Chen Guanke

来源：Chinese Journal of Chemistry, 2009, 27(10): 2090-2092.

DOI：10.1002/cjoc.200990351

• dynorphin A
• PEGylation degree
• molar ratio
• pH

Dynorphin A (1-13) was chemically modified by reaction with succinimidyl propionate-monomethoxy polyethylene glycol (mPEG-SPA). To determine the degree and the optimized condition for PEGylation of dynorphin A, the reactions were monitored in different pH buffers at different molar ratios by reversed-phase high performance liquid chromatography (RP-HPLC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The results showed that the degree of PEGylation for dynorphin A could easily be controlled through adjustment of the molar ratios and the pH. The degree of PEGylation of dynorphin A increased as the molar ratio of dynorphin to MPEG-SPA and the pH increased.

• 出版日期2009-10
• 单位广东药科大学