Comparison of immunochromatography, PCR and culture methods for the detection of Campylobacter bacteria

作者：Dey Shuvra Kanti; Nishimura Shuichi; Okitsu Shoko; Hayakawa Satoshi; Mizuguchi Masashi; Ushijima Hiroshi^*

来源：Journal of Microbiological Methods, 2012, 91(3): 566-568.

DOI：10.1016/j.mimet.2012.09.034

• Immunoenzymatic
• Rapid detection
• PCR
• Culture methods
• Campylobacter jejuni

A total of 463 fecal specimens from human patients were tested for the presence of Camphylobacter by a commercial immunochromatography kit (ImmunoCard STAT! CAMPY), polymerase chain reaction (PCR) and conventional bacteriological (CB) methods. Using culture as the standard of reference, the ImmunoCard STAT! CAMPY assay had a sensitivity of 86% and a specificity of 100%. On the other hand, using PCR as the standard of reference, ImmunoCard STAT! CAMPY assay had a sensitivity of 90.5% and a specificity of 100%.

• 出版日期2012-12