A high performance liquid chromatography with ultraviolet method for Eschweilera nana leaves and their anti-inflammatory and antioxidant activities

作者：Outuki Priscila M; Lazzeri Nides S; de Francisco Lizziane M B; Bersani Amado Ciomar A; Ferreira Izabal C P; Cardoso Mara Lane C^*

来源：Pharmacognosy Magazine, 2015, 11(43): 619-626.

DOI：10.4103/0973-1296.160464

• Antioxidant activity
• Eschweilera nana Miers
• flavonoids
• phytochemical composition
• topic anti-inflammatory

Background: Eschweilera nana Miers is a tree widely distributed in Cerrado, Brazil. Objective: In this study, we aimed to describe its phytochemical properties and antioxidant and topical anti-inflammatory effects for the first time, as well validate an high performance liquid chromatography with ultraviolet/visible (HPLC-UV-Vis) method for the separation and quantification of the main components (hyperoside and rutin) in the hydroalcoholic extract of E. nana leaves. Materials and Methods: Structural identification of compounds in E. nana extract was performed by analysis of spectral data by H-1 nuclear magnetic resonance, C-13 nuclear magnetic resonance and/or ESI/EM. The HPLC-UV-Vis method was validated according International Conference on Harmonization (ICH) parameters. The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) method were used for determination of in vitro antioxidant activities and the croton oil-induced inflammationt for evaluation of in vivo anti-inflammatory effects. Results: Hyperoside, rutin,alpha-amirin, beta-sitosterol, and stigmasterol were identified in the hydroalcoholic extract of E. nana leaves. HPLC-UV-Vis was validated according to ICH parameters. Furthermore, in vitro and in vivo assays demonstrated that the hydroalcoholic extract and methanol traction showed significant antioxidant and topical anti-inflammatory effects, as they were able to reduce ear edema induced by croton-oil application. Conclusions: This research showed the first phytochemical study:of E. nana extract and their biological activities may be associated with the presence of flavonoids in the extracts.

• 出版日期2015-9