Macrophage polarization, inflammatory signaling, and NF-kappa B activation in response to chemically modified titanium surfaces

作者：Zhang, Hao; Wu, Xuelin; Wang, Guangchao; Liu, Peizhao; Qin, Sheng; Xu, Kaihang; Tong, Dake; Ding, Hong; Tang, Hao^*; Ji, Fang^*

来源：Colloids and Surfaces B: Biointerfaces , 2018, 166: 269-276.

DOI：10.1016/j.colsurfb.2018.03.029

• Titanium
• Surface modification
• Antibacterial
• Pro-inflammatory response
• Macrophage polarization
• THP-1 macrophage

Functionalization of titanium devices with various bioactive molecules enhances many of their properties as implants, including biocompatibility, which is typically assessed by macrophage activation and inflammation. However, functionalization requires prior introduction of reactive groups, to which bioactive agents can then be grafted. Thus, we investigated the inflammatory properties of titanium pretreated with NaOH, titanium pretreated with NaOH and then with 3-aminopropyl triethoxysilane, and titanium pretreated with dopamine. Inflammation, macrophage polarization, and activation of NF-kappa B signaling were assessed by real-time PCR and western blotting. The data demonstrate that silanized titanium is the least inflammatory, and promotes macrophage M2 polarization with modest engagement of the NF-kappa B signaling pathway. Importantly, silanization introduces a reactive amino group, providing more opportunities for further functionalization.

• 出版日期2018-6-1
• 单位中国人民解放军第二军医大学; 上海市徐汇区大华医院