ANALYSIS OF IMMUNOGLOBULIN GENE REARRANGEMENT IN PARAFFIN-EMBEDDED TISSUES BY POLYMERASE CHAIN-REACTION (PCR)

作者：DUBUS P^*; LABOUYRIE E; BILHOUNABERA C; BELLEANNEE G; VERGIER B; BERNARD P; DEMASCAREL A; MERLIO JP

来源：Annales de Pathologie, 1994, 14(4): 227-233.

Family-specific primers were used in polymerase chain reaction (PCR) to analyse clonality of immunoglobulin heavy chain (IgH) gene rearrangement. DNA templates were extracted from formalin-fixed paraffin-embedded biopsies from B-cell lymphomas (n=19), T-cell lymphomas (n=3), reactive lymphadenopathies (n=20) and negative controls (n=2). PCR was also performed on DNA extracted from fragments of the same biopsies that were either fixed in Bouin's solution (n=24) or snap-frozen (n=22). The latter were also studied for IgH gene rearrangement by the Southern blot technique. Polyclonal or clonal fragments were obtained from all frozen biopsies. No amplification was observed in 3 out of 44 formalin fixed specimens and 18 out of 24 Bouin fixed specimens. A clonal amplification was only observed in 16 out o 19 formalin-fixed B-cell lymphoma specimens. Therefore this technique allows to study B-cell clonality from formalin-fixed, embedded material.

• 出版日期1994