A Uniquely Configured Acetylcholinesterase-Lecithin Biomimetic Sensing Structure at Cyclohexane/Water Interface for Electrochemical Determination of Fenthion in Organic Solvent

作者：Li Jun; He Jingjing; Qiu Jingxia; Zhang Shanqing; Zhao Huijun; Miao Yuqing^*

来源：Electroanalysis, 2010, 22(10): 1061-1065.

DOI：10.1002/elan.200900529

• Acetylcholinesterase
• Lecithin
• Biomimetic
• Two phases
• Square wave voltammetry
• Interfaces

An acetylcholinesterase (AChE)-lecithin biomimetic structure was constructed at the oil/water interface for the direct determination of fenthion in cyclohexane. Indophenol acetate in oil phase was hydrolyzed by AChE at the two-phase interface to produce indophenol. Square wave voltammetry was used to monitor the current decrease of substrate to assess AChE activity. The AChE incorporated in the lecithin-based biomimetic layer possessed higher enzymatic activity and was more sensitive to fenthion inhibition than freestanding AChE. A linear relationship between the inhibition percentage and logarithm of fenthion concentration was obtained in a concentration range from 1 ng/mL to 1 mg/mL.

• 出版日期2010-5
• 单位浙江师范大学