A Combined Peritoneal Macrophage/Cell Membrane Chromatography and Offline GC-MS Method for Screening Anti-Inflammatory Components from Chinese Traditional Medicine Houttuynia cordata Thunb.

作者：Li Weifeng; Niu Xiaofeng; Zhou Ping; Li Miao; He Langchong^*

来源：Chromatographia, 2011, 73(7-8): 673-680.

DOI：10.1007/s10337-011-1926-5

• Cell membrane chromatography
• Gas chromatography-mass spectrometry
• Methyl nonyl ketone
• Peritoneal macrophage
• Anti-inflammatory

The aim of this study was to screen for the anti-inflammatory components from Houttuynia cordata Thunb (HCT) using an analytical method combining cell membrane chromatography (CMC) with gas chromatography-mass spectrometry (GC-MS). The stationary phase of the CMC employed mouse peritoneal macrophage (PM) cell membranes. We investigated the performance of the PM/CMC-offline-GC-MS method using hydrocortisone (HC) as standard. The method was then applied to the identification of anti-inflammatory components in extracts of HCT. The major component retained by CMC was identified as methyl nonyl ketone (MNK) by GC-MS. In vitro experiments revealed that MNK was able to inhibit LPS-induction of TNF-alpha, NO, and H(2)O(2) production in a dose-dependent manner. IC(50) values were 3.3, 4.1, and 3.6 mu g mL(-1), respectively. The PM/CMC-offline-GC-MS method is an effective screening system for the rapid detection, enrichment, and identification of target components from complex samples.

• 出版日期2011-4
• 单位西安交通大学