MitoNeoD: A Mitochondria-Targeted Superoxide Probe

作者：Shchepinova Maria M; Cairns Andrew G; Prime Tracy A; Logan Angela; James Andrew M; Hall Andrew R; Vidoni Sara; Arndt Sabine; Caldwell Stuart T; Prag Hiran A; Pell Victoria R; Krieg Thomas; Mulvey John F; Yadav Pooja; Cobley James N; Bright Thomas P; Senn Hans M; Anderson Robert F; Murphy Michael P^*; Hartley Richard C^*

来源：Cell Chemical Biology, 2017, 24(10): 1285-+.

DOI：10.1016/j.chembiol.2017.08.003

Mitochondrial superoxide (O-2(center dot-)) underlies much oxidative damage and redox signaling. Fluorescent probes can detect O-2(center dot-), but are of limited applicability in vivo, while in cells their usefulness is constrained by side reactions and DNA intercalation. To overcome these limitations, we developed a dual-purpose mitochondrial O-2(center dot-) probe, MitoNeoD, which can assess O-2(center dot-) changes in vivo by mass spectrometry and in vitro by fluorescence. MitoNeoD comprises a O-2(center dot-)-sensitive reduced phenanthridinium moiety modified to prevent DNA intercalation, as well as a carbon-deuterium bond to enhance its selectivity for O-2(center dot-) over non-specific oxidation, and a triphenylphosphonium lipophilic cation moiety leading to the rapid accumulation within mitochondria. We demonstrated that MitoNeoD was a versatile and robust probe to assess changes in mitochondrial O-2(center dot-) from isolated mitochondria to animal models, thus offering away to examine the many roles of mitochondrial O-2(center dot-) production in health and disease.

• 出版日期2017-10-19