Use of different molecular typing methods for the study of heterogeneity within Clostridium difficile toxinotypes V and III

作者：Janezic S; Indra A; Allerberger F; Rupnik M^*

来源：Journal of Medical Microbiology, 2011, 60(8): 1101-1107.

DOI：10.1099/jmm.0.031054-0

Clostridium difficile strains of toxinotypes III (n=13) and V (n=45) were typed by agarose gel-based PCR ribotyping, capillary gel electrophoresis-based PCR ribotyping and PFGE using two different restriction enzymes, Smal and Sacll. With conventional agarose gel-based PCR ribotyping, toxinotype III strains were distributed among six different PCR ribotypes and toxinotype V strains into three different PCR ribotypes. Capillary gel electrophoresis-based ribotyping was more discriminatory for toxinotype V strains, with six different ribotypes found. With PFGE using Smal, all toxinotype Ill strains grouped together into a single pulsotype. Using Sacll, ribotype 027 strains grouped together with > 90% similarity and were < 83% similar to other ribotypes of toxinotype Ill strains. Within ribotype 078, seven (Smal) and eight (Sad) different pulsotypes were found, whilst ribotype 126 strains belonged to one (Smal) and two (Sacll) pulsotypes. Within ribotype 066, it was possible to distinguish between pig and human isolates. Using Sad, a further distinction could also be made between pig isolates from two different farms. PFGE (Smal and Sacll) clustered strains according to their toxinotype; however, correlation of PFGE and ribotyping was better with Sad. These data suggest that toxinotype Ill strains are a more heterogeneous group than toxinotype V strains and that Sacll is more discriminatory than Smal. Alternatively, the use of both enzymes simultaneously could improve PFGE typing of C. difficile.

• 出版日期2011-8
• 单位河北医科大学