An ultrasensitive and ultraselective chemiluminescence aptasensor for dopamine detection based on aptamers modified magnetic mesoporous silica @ graphite oxide polymers

作者：Sun, Yuanling; Lin, Yanna; Ding, Chaofan; Sun, Weiyan; Dai, Yuxue; Zhu, Xiaodong; Liu, Hao; Luo, Chuannan^*

来源：Sensors and Actuators B: Chemical , 2018, 257: 312-323.

DOI：10.1016/j.snb.2017.10.171

• Chemiluminescence
• Aptasensor
• Mesoporous silica
• Graphene oxide
• AuNPs
• Da

In this work, an ultrasensitive and ultraselective chemiluminescence (CL) aptasensor was prepared for dopamine (DA) detection based on aptamers modified magnetic mesoporous silica @ graphite oxide polymers (Fe3O4 center dot mSiO(2)center dot nSiO(2)@GO). Firstly, Fe3O4 center dot mSiO(2)center dot nSiO(2)@GO and Au nanoparticles (AuNPs) were prepared and characterizated by transmission electron microscopy (TEM), scanning electron microscope (SEM), fourier transform infrared (FTIR), X-ray powder diffraction (XRD) and others. Then, dopamine aptamer (D-Apt) was immobilized on the surface of Fe3O4 center dot mSiO(2)center dot nSiO(2)@GO while AuNPs was modified by ssDNA (a single stranded DNA partially complementary to D-Apt). The immobilization properties of Fe3O4 center dot mSiO(2)center dot nSiO(2)@GO to D-Apt and the adsorption properties of Fe3O4 center dot mSiO(2)center dot nSiO(2)@GO@D-Apt to AuNPs@ssDNA were researched sequentially. When DA existed, AuNPs@ssDNA was desorbed from the surface of Fe3O4 center dot mSiO(2)center dot nSiO(2)@GO@D-Atp and catalyzed luminescence. After that, under optimized CL conditions, DA could be measured with the linear concentration range of 2.5 x 10(-13) to 2.0 x 10(-9) mol/L. The detection limit was 3.9 x 10(-14) mol/L (3 delta) while the relative standard deviation (RSD) was 2.6%. Finally, the Fe3O4 center dot mSiO(2)center dot nSiO(2)@GO@D-Apt/AuNPs@ssDNA - CL aptasensor was used for the determination of DA in practical samples and recoveries ranged from 98.0% to 102.0%. Those satisfactory results clarified the proposed CL aptasensor achieved ultraselective, ultrasensitive and reliable detection of DA and revealed potential application in monitoring and diagnosis of human neurological diseases.

• 出版日期2018-3
• 单位济南大学