A novel hydrogen peroxide sensor based on immobilization of haemoglobin on nano-TiO2/DTAB composite film

作者：Zhou, Yuanzhen^*; Zhang, Haiyan; Chen, Bin; Wang, Hui

来源：Biocatalysis and Biotransformation, 2012, 30(4): 377-384.

DOI：10.3109/10242422.2012.701622

• hydrogen peroxide sensor
• haemoglobin
• Nano-TiO2
• dodecyltrimethylammonium bromide

The direct electron transfer of immobilized haemoglobin (Hb) on nano-TiO2 and dodecyltrimethylammonium bromide (DTAB) film modified carbon paste electrode (CPE) and its application as a hydrogen peroxide (H2O2) biosensor were investigated. On nano-TiO2/DTAB/Hb/CPE, Hb displayed a rapid electron transfer process with participation of one proton and with an electron transfer rate constant which estimated as 0.29 s(-1). Thus, the proposed biosensor exhibited a high sensitivity and excellent electrocatalytic activity for the reduction of H2O2. The catalytic reduction current of H2O2 was proportional to H2O2 concentration in the range of 0.2-4.0 mM with a detection limit of 0.07 mM. The apparent Michaelis-Menten constant (K-m(app)) of the biosensor was calculated to be 0.127 mM, exhibiting a high enzymatic activity and affinity. This sensor for H2O2 can potentially be applied in determination of other reactive oxygen species as well.

• 出版日期2012-8
• 单位西安建筑科技大学