Granulocyte colony-stimulating factor improves the efficacy of autologous bone marrow-derived mononuclear cell transplantation treatment for lower limb ischemia

作者：Gu, Yongquan^*; Guo, Lianrui; Guo, Jianming; Dardik, Alan; Zhang, Shuwen; Tong, Zhu; Zhang, Jian; Wang, Zhonghao

来源：International Angiology, 2017, 36(4): 346-353.

DOI：10.23736/S0392-9590.16.03796-2

• Granulocyte colony-stimulating factor
• Bone marrow
• Ischemia

BACKGROUND: The safety and efficacy of autologous bone marrow-derived mononuclear cell (BM-MNC) transplantation in the treatment of lower limb ischemia is becoming established, although common treatment protocols are not yet agreed upon. We hypothesized that bone marrow mobilization with granulocyte colony-stimulating factor (G-CSF) improves the safety and effectiveness of cellular therapy.
METHODS: Forty-four patients were randomly assigned to receive two injections of G-CSF (300 mu g) prior to BM-MNC transplantation. BM-MNC were harvested from all patients and injected as equal aliquots of at least 10(8) cells into the ischemic leg muscles below the lowest patent artery.
RESULTS: After 3 months, patients receiving G-CSF reported increased subjective relief of symptoms and showed increased transcutaneous oxygen tension (TcPO2). After 6 months, patients showed greater improvement in TcPO2, ankle-brachial index, and angiographic score compared to control patients. There were no increased numbers of side effects in patients receiving G-CSF.
CONCLUSIONS: G-CSF is safe and effective to mobilize BM-MNC and may allow reduced volume of aspirated bone marrow, potentially reducing procedural complications. G-CSF should be considered for use in patients that are candidates for angiogenic therapy. G-CSF may increase the number of patients that are candidates for therapeutic angiogenesis.

• 出版日期2017-8
• 单位首都医科大学