A Theiler's virus BeAn strain shows a persistent profile of BHK-21 cell infection as determined by genome detection using real-time RT-PCR and expression of L* protein

作者：Velloso Alvaro Jorge; da Silva Junior Haroldo Cid; Santos Eneida Almeida; Baroni Eliane Barbosa; Baroni de Moraes Marcia Terezinha

来源：Archives of Virology, 2012, 157(12): 2437-2440.

DOI：10.1007/s00705-012-1434-4

Cells from various tissues and species are able to bind to Theiler's virus strain DA and allow it to replicate to some extent. Meanwhile, permissiveness in vitro to BeAn strains has not been well investigated. In this paper, the BeAn 8386 virus was subjected to five passages in BHK-21 cells and showed a persistent profile. In order to follow the in vitro infection, real-time RT-PCR to detect the IRES, L* and 3A3B regions of the Theiler's virus genome was carried out in the first and last passages. In addition, the expression of L* protein was detected. These findings confirm the persistence of the virus in vitro, even in the absence of cytopathic effect (CPE).

• 出版日期2012-12