A selenium-containing single-chain abzyme with potent antioxidant activity

作者：You DL; Ren XJ; Xue Y; Luo GM^*; Yang TS; Shen JC

来源：European Journal of Biochemistry, 2003, 270(21): 4326-4331.

DOI：10.1046/j.1432-1033.2003.03825.x

• antioxidant activity
• glutathione peroxidase
• mitochondria
• scFv
• selenium

Reactive oxygen species (ROS) are products of normal metabolic activities and are thought to be the cause of many diseases. A selenium-containing single-chain abzyme 2F3 (Se-2F3-scFv) that imitates glutathione peroxidase has been produced which has the capacity to remove ROS. To evaluate the antioxidant ability of Se-2F3-scFv, we constructed a ferrous sulfate/ascorbate (Vc/Fe2+)-induced mitochondrial damage model system and investigated the capacity of Se-2F3-scFv to protect mitochondria from oxidative damage. Se-2F3-scFv markedly decreased mitochondrial swelling, inhibited lipid peroxidation, and maintained the activity of cytochrome c oxidase, in comparison with Ebselen, a well-studied glutathione peroxidase mimic, indicating that Se-2F3-scFv has potential for treating diseases mediated by ROS.

• 出版日期2003-11
• 单位吉林大学