Analysis of genetic variability in regenerated and germinated plantlets of ten indica rice genotypes (Oryza sativa L.) using RAPD markers

作者：Dey R C; Hassan L^*; Al Munsur M A Z; Islam M M; Begum S N; Halder J

来源：Agrochimica, 2012, 56(1): 55-64.

• Genetic variation
• rice
• Oryza sativa
• RAPD

RAPD assay was tried for molecular analysis on regenerated and germinated genotypes of 10 rice genotypes namely BRRI dhan28, BRRI dhan29, BRRI dhan32, BRRI dhan36, BRRI dhan40, BRRI dhan41, BRRI dhan45, BRRI dhan47, Binadhan-6 and Haridhan to observe genetic variation among them. Three primers out of 10 produced a total of 35 bands of which 29 bands considered as polymorphic and the polymorphism per cent was 82.86. The primer G7 produced the highest per cent of polymorphic bands (84.61%), whereas OPF 15 produced the lowest (80%). The highest level of gene diversity value (0.5000) and Shannon's index (0.6931) were found in loci OPA 20-3, OPA 20-11 and G7-9, while, the lowest levels were found in OPA 20-8 and OPF15-10 (0.0950 and 0.1985) respectively. Dendrogram based on UPGMA indicated segregation of the 20 rice genotypes into two main clusters. BRRI dhan36 control and Binadhan-6 control were distinctly located in the dendrogram which supports the highest genetic distance (0.9904) between them. In cluster I, Binadhan-6 control and Haridhan regenerated were grouped in same sub cluster and also showed the lowest genetic distance (0.1214) between them.

• 出版日期2012-2