Molecular cloning, expression and oxidative stress response of the vitellogenin Gene (AccVg) from Apis cerana cerana

作者：Zhang, Weixing; Liu, Zhenguo; Zhu, Ming; Ma, Lanting; Wang, Ying; Wang, Hongfang; Guo, Xingqi; Xu, Baohua^*

来源：Apidologie, 2017, 48(5): 599-611.

DOI：10.1007/s13592-017-0503-9

• Vitellogenin
• Apis cerana cerana
• RNA interference
• Oxidative stress response
• RT-PCR

Vitellogenin (Vg) is a yolk precursor protein in most oviparous females. However, Vg has not been studied in the Apis cerana cerana. In this work, the Vg gene of the A. cerana cerana has been cloned and sequenced. The gene codes for a protein consisting of 1770 amino acids in seven exons with a predicted molecular mass and isoelectric point of 200 kDa and 6.46, respectively. Additionally, an 807-bp 5'-flanking region was isolated, and potential transcription factor binding sites associated with development and stress response were identified. Quantitative real-time PCR analysis showed that AccVg is highly expressed in pupae during different developmental stages. In addition, the expression of AccVg could be induced by cold (4 and 16 A degrees C), CdCl2, and pesticide treatment. Real-time quantitative PCR (RT-PCR) and western blot analyses indicated that AccVg transcription was induced by various abiotic stresses. Western blot was used to measure the expression levels of AccVg protein. Taken together, these results suggest that AccVg most likely plays essential roles in antioxidant defence and that it may be of critical importance to honey bee survival.

• 出版日期2017-9
• 单位山东农业大学